Part:BBa_K5301024:Design
Multi-polymer MSP, which refers to large circular MSPs through end-to-end connections of multiple MS
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3141
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3141
Illegal NotI site found at 1720
Illegal NotI site found at 4038 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1239
Illegal BglII site found at 2323
Illegal BglII site found at 2695
Illegal BglII site found at 3557
Illegal BglII site found at 3929 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3141
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3141
Illegal AgeI site found at 1035
Illegal AgeI site found at 1828
Illegal AgeI site found at 2220
Illegal AgeI site found at 2980 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We carefully designed the joints of three MSP segments to ensure that a single MSP does not undergo self-cyclization or dimerization, but can form the correct multi-polymerized MSP. Finally, mCherry emits red fluorescence after covalent interaction occurs at the last pair of linkers-SpyCatcher/Tag, indicating the successful construction of MSP.
Source
SpyCatcher/Tag are derived from Streptococcus pyogenes fibronectin-binding protein, FbaB. SdyCatcher/Tag are derived from Streptococcus dysgalactiae protein, CnaB. SnoopCatcher/Tag are derived from RrgA, an adhesin from Streptococcus pneumoniae. mCherry [1-10] and [11] come from mCherry fluorescent protein. NW15 is the truncated form of NW30, which is originated from a set of α-helixes derived from apolipoprotein A-1 (ApoA1).